Table 1 Nucleotide sequences of primers and operating condition for PCR analysis
Gene | Nucleotide sequence | Operating condition | |
|---|---|---|---|
GAPDH | sense | 5′-AACTTTGGCATTGTGGAAGG-3′ | 94 °C, 30s 58 °C, 30s 72 °C, 30s, 30 cycles |
antisense | 5′-ACACATTGGGGGTAGGAACA-3′ | ||
IL-1β | sense | 5′-GGCTGTGGAGAAGCTGTGGC-3′ | |
antisense | 5′-GGGTGGGTGTGCCGTCTTTC-3′ | ||
TNF-α | sense | 5′-GCAGAAGAGGCACTCCCCCA-3′ | |
antisense | 5′-GATCCATGCCGTTGGCCAGG-3′ | ||
IL-4 | sense | 5′-TCAACCCCCAGCTAGTTGTC-3′ | |
antisense | 5′-TGTTCTTCGTTGCTGTGAGG-3′ | ||