Fig. 8

Effects of FNP on phosphorylation of MAPKs and AMPK in LPS-stimulated RAW264.7 cells. Notes: The cells were pretreated with different concentrations (40 μg/mL and 80 μg/mL) of FNP for 2 h or 4 h and stimulated with 1 μg/ml of LPS for 1 h. Total cellular proteins (50 μg) were separated by SDS-PAGE, then transferred to PVDF membrane and detected by Western blot analysis. Western blot results were shown in (a). Quantification of p-AMPKα, p-ERK1/2, p-P38, p-JNK and p-c-JUN protein expression was normalized to AMPKα, ERK1/2, P38, JNK and c-JUN using a densitometer. Each column represented as the means ± SD from three independent experiments. Results of 4h pretreatment were shown in (b) and results of 2 h pretreatment were shown in (c). _# P <0.05 vs control group; _##P <0.01 vs control group; _* P <0.05 vs LPS group; _** P <0.01 vs LPS group