Fig. 3

Effects of FNP pretreatment on TNF-α levels in blood and tissues, mRNA expression in lung. Notes: The mice in the LPS group were injected intraperitoneally with 17 mg/kg (body weight) of LPS, while control group were injected with PBS (10 mL/kg). FNP50, FNP100 and FNP200 group were orally administrated with diverse dose FNP (50 mg/kg, 100 mg/kg and 200 mg/kg) for 3 days before instillation of LPS. Serum and lung were collected, liver and intestine homogenates were prepared. TNF-α levels of serum and homogenates were shown in (a). The level of mRNA expression in lung is expressed as fold-change relative to the control group (b). The mRNA expression of TNF-α, IFN-α, IFN-γ and IL-2 was analyzed by qRT-PCR. Data are presented as the means ± SD from three independent experiments (n = 10). _## P <0.01 vs control group; _* P <0.05 vs LPS group; _** P <0.01 vs LPS group