Table 1 Condition for LC-MS/MS analysis of Licorice extracts
A. | |
HPLC Condition | |
Column | Luna C18 RP column (2.0 × 150 mm, 5 μm) |
Flow rate | 0.3 mL/min |
Injection volume | 5 μL |
Column temperature | 40 °C |
Autosampler temperature | 4 °C |
B. | |
Mass condition | |
Ion source | Turbo spray (Negative) |
Curtain Gas | 30 psi |
Collision Gas | N2 (Medium) |
Ionspray Voltage | - 4.0 kV |
Source temperature | 400 °C |
Gas 1 | 40 psi |
Gas 2 | 50 psi |