Fig. 2

Effect of butein on serum-stimulated B16F10 cell migration. a B16F10 cells (2 × 10⁴) cultured in serum-free DMEM were pretreated with or without butein for 30 minutes, and placed in the upper chamber of a Transwell containing with 0.2 % gelatin-coated filter membrane. DMEM with 10 % FBS was added to the lower chamber. After removal of non-migrated cells and fixation, cells that migrated to the underside filter membrane were stained and quantified by phase-contrast light microscope under a high-power field (HPF; magnification, 100×). Three fields per filter were counted at 100× magnification. The quantitative of migratory cell was shown in b. Data are expressed as mean ± S.E.M. of five independent experiments. *p < 0.05; **p < 0.01 compare with the control (Veh) group