|
Antibioticsa
|
Extract and concentration
|
Bacterial strainsb, MIC (μg/mL) of antibiotics in the absence and presence of the extract and FIC in parenthesis
|
PBSS (%)
|
|---|
| | |
AG102
|
AG100ATET
|
EA27
|
CM64
|
KP55
|
NAE16
|
BM67
|
PA01
|
PA124
| |
|---|
|
CIP
| |
0
|
4
|
64
|
4
|
64
|
4
|
128
|
32
|
16
|
32
| |
| |
L
|
MIC/2
|
4 (1)I
|
64 (1)I
|
1 (0.25)S
|
64 (1)I
|
8 (2)A
|
128 (1)I
|
16 (0.50)S
|
16 (1)I
|
16(0.50)S
|
3/9 (27.27 %)
|
| | |
MIC/5
|
4 (1)I
|
64 (1)I
|
2 (0.50)S
|
64 (1)I
|
8 (2)A
|
128 (1)I
|
32 (1)I
|
16 (1)I
|
32(1)I
|
1/9 (11.11 %)
|
| |
B
|
MIC/2
|
4 (1)I
|
32 (0.50)S
|
1 (0.25)S
|
64 (1)I
|
4 (1)I
|
128 (1)I
|
16 (0.50)S
|
16 (1)I
|
32(1)I
|
3/9 (27.27 %)
|
| | |
MIC/5
|
4 (1)I
|
64 (1)I
|
2 (0.50)S
|
64 (1)I
|
4 (1)I
|
128 (1)I
|
32 (1)I
|
16 (1)I
|
32(1)I
|
3/9 (27.27 %)
|
|
TET
| |
0
|
8
|
64
|
64
|
32
|
2
|
64
|
32
|
64
|
16
| |
| |
L
|
MIC/2
|
2 (0.25)S
|
32 (0.50)S
|
32 (0.50)S
|
8 (0.25)S
|
2 (1)I
|
32 (0.50)S
|
32 (1)I
|
16 (0.25)S
|
8(0.50)S
|
7/9 (77.78 %)
|
| | |
MIC/5
|
4 (0.50)S
|
64 (1)I
|
64 (1)I
|
16 (0.50)S
|
2 (1)I
|
64 (1)I
|
32 (1)I
|
64 (1)I
|
8(0.50)S
|
3/9 (27.27 %)
|
| |
B
|
MIC/2
|
4 (0.50)S
|
32 (0.50)S
|
16 (0.25)S
|
16 (0.50)S
|
1 (0.50)S
|
16 (0.25)S
|
16 (0.50)S
|
32 (0.50)S
|
8(0.50)S
|
9/9 (100 %)
|
| | |
MIC/5
|
4 (0.50)S
|
64 (1)I
|
64 (1)I
|
16 (0.50)S
|
1 (0.50)S
|
32 (0.50)S
|
32 (1)I
|
64 (1)I
|
16(1)I
|
4/9 (36.36 %)
|
|
KAN
| |
0
|
-
|
16
|
128
|
4
|
16
|
16
|
64
|
4
|
128
| |
| |
L
|
MIC/2
|
8(˂0.03)S
|
4 (0.25)S
|
64 (0.50)S
|
2 (0.50)S
|
16 (1)I
|
16 (1)I
|
32 (0.50)S
|
4 (1)I
|
64(0.50)S
|
6/9 (66.67 %)
|
| | |
MIC/5
|
64 (˂0.25)S
|
16 (1)I
|
128 (1)
|
4 (1)I
|
16 (1)I
|
16 (1)I
|
64 (1)I
|
2 (0.50)S
|
64(0.50)S
|
3/9 (27.27 %)
|
| |
B
|
MIC/2
|
8 (˂0.03)S
|
˂1 (˂0.06)S
|
32 (0.25)S
|
˂1 (˂0.25)S
|
<1 (<0.06)S
|
8 (0.50)S
|
16 (0.25)S
|
4 (1)I
|
32(0.25)S
|
8/9 (88.89 %)
|
| | |
MIC/5
|
128 (˂0.50)S
|
8 (0.50)S
|
128 (1)I
|
2 (0.50)S
|
4 (0.25)S
|
16 (1)I
|
32 (0.50)S
|
<1 (<0.25)S
|
64(0.50)S
|
6/9 (66.67 %)
|
|
AMP
| |
0
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
-
| |
| |
L
|
MIC/2
|
128 (˂0.50)S
|
128 (˂1)S
|
-(≥1)
|
-(≥1)
|
-(≥1)
|
- (≥1)
|
-(≥1)
|
-(≥1)
|
-(≥1)
|
2/9 (22.22 %)
|
| | |
MIC/5
|
256 (˂1)S
|
-(≥1)
|
-(≥1)
|
-(≥1)
|
-(≥1)
|
- (≥1)
|
-(≥1)
|
128 (<0.50)S
|
-(≥1)
|
2/9 (22.22 %)
|
| |
B
|
MIC/2
|
128 (˂0.50)S
|
-(≥1)
|
256 (˂1)S
|
-(≥1)
|
256 (<1)S
|
256 (<1)S
|
-(≥1)
|
-(≥1)
|
256(<1)S
|
5/9 (55.55 %)
|
| | |
MIC/5
|
256 (˂1)S
|
-(≥1)
|
-(≥1)
|
-(≥1)
|
-(≥1)
|
- (≥1)
|
-(≥1)
|
256 (˂1)S
|
-(>1)
|
2/9 (22.22 %)
|
|
CHL
| |
0
|
32
|
64
|
64
|
-
|
8
|
64
|
128
|
16
|
128
| |
| |
L
|
MIC/2
|
16 (0.50)S
|
32 (0.50)S
|
64 (1)I
|
128 (˂0.50)S
|
16 (2)A
|
8 (0.13)S
|
64 (0.50)S
|
2 (0.13)
|
64(0.50)S
|
6/9 (66.67 %)
|
| | |
MIC/5
|
32 (1)I
|
64 (1)I
|
64 (1)I
|
-(˃1)
|
16 (2)A
|
16 (0.25)S
|
64 (0.50)S
|
8 (0.50)S
|
128(1)I
|
3/9 (27.27 %)
|
| |
B
|
MIC/2
|
32 (1)I
|
32 (0.50)S
|
32 (0.50)S
|
256 (˂1)S
|
2 (0.25)S
|
16 (0.25)S
|
64 (0.50)S
|
4 (0.25)S
|
64(0.50)S
|
8/9 (88.89 %)
|
| | |
MIC/5
|
32 (1)I
|
32 (0.50)S
|
64 (1)I
|
-(˃1)
|
2 (0.25)S
|
32 (0.50)S
|
128 (1)I
|
8 (0.50)S
|
64(0.50)S
|
5/9 (55.55 %)
|
- aAntibotics [TET tetracycline, CIP ciprofloxacin, KAN kanamycin, CHL chloramphenicol, AMP ampicillin]. bBacterial strains: Escherichia coli [AG102, AG100Atet], Pseudomonas aeruginosa [PA01, PA124], Enterobacter aerogenes [CM64, EA27], Enterobacter cloacae [BM67], Klebsiella pneumoniae [KP55], Providencia stuartii [NAE16]. cPBSS: percentage of bacteria strain on which synergism has been observed
- (): fold increase in MIC values of the antibiotics after association with plants extract; S synergy, I indifference, na not applicable, B bart extract, L leaves extract, FIC fractional inhibitory concentration, (−): >256 μg/mL; 0: no extract (only antibiotic tested)
