Fig. 6

Apoptotic morphology detection by Acridine orange-ethidium bromide (AO/EB) fluorescent staining of RD cell line treated with the MEFCA. a-negative control; b- cycloheximide as the positive control (5 mM; 50 μL); c- 20 μg/mL, d- 50 μg/mL of the extract. Green arrows: live cells, greenish yellow: early apoptotic cells, orange red: late apoptotic cells, red: dead cells (some cells are fragmented and become faded). This figure denotes the results of at least 3 independent experiments (Original magnification 40×)