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Table 7 Percentage of viable, apoptotic and dead cells analysed by flow cytometry

From: Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action

Cells (%) PEF (μg/ml) Control+ S.E.M
0 5 10 20 40
Viable 15.2d 16.2d 27.3c 36.1b 46.2a 40.2ab 3.2
Apoptotic 52.8a 54.1a 48.4b 43.4bc 37.1d 41.1cd 2.4
Dead 32.1a 29.8a 24.3b 20.7bc 16.9d 18.8cd 2.1
  1. The cells were pre-treated with different concentrations of PEF for 24 h, then the media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h
  2. A minimum of 15,000 cells per sample was analysed by flow cytometry
  3. Control+: 10 μM or 1.7 μg/ml gallic acid
  4. Means with different superscripts (a,b,c,d) within a row are significantly different (p < 0.05)
  5. PEF Phenolic-enriched fraction
  6. S.E.M Standard error of the means