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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: The Chinese medicine Sini-San inhibits HBx-induced migration and invasiveness of human hepatocellular carcinoma cells

Fig. 1

Establishment of constitutive HBx expression in HepG2 cells. HepG2 cells were transfected with pRT-HBx-GFP. After selection of G418-resistant colonies, cell proliferation with and without DOX treatment was assessed by MTT assay (a). HepG2-HBx-GFP cells with and without DOX were observed with a fluorescence microscope (b). Expression of the HBx-GFP fusion protein in HepG2-HBx-GFP cells with and without DOX treatment was determined by Western blot analysis using a GFP-specific antibody. β-actin was included as an internal control (c)

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