Fig. 2

S. hexaphylla suppresses RANKL-induced ERK, NF-κB, and Ca2+ signaling pathways. BMMs were starved with serum-free α-MEM media for 3 h. BMMs were pretreated with S. hexaphylla for 1 h and then stimulated with RANKL for the indicated times. BMMs were pretreated with or without S. hexaphylla for 1 h and then stimulated with RANKL (100 ng/mL) for the indicated time. The cell lysates were analyzed by western blotting with antibodies. β-actin was used as the internal control