Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model

Table 2 Comparison of relative organ weights and biochemical parameters in DBA/1J mice with CIA

Groups (n = 10)		Normal	Control	MTX	Brazilin
Average relative organ weights (organ weight/body weight, %)
	Liver	4.93 ± 0.10	6.01 ± 0.48*	4.84 ± 0.62	6.03 ± 0.72
	Kidney	1.98 ± 0.11	1.95 ± 0.32	1.63 ± 0.11*	1.92 ± 0.13
	Spleen	0.25 ± 0.02	0.48 ± 0.14*	0.34 ± 0.10	0.56 ± 0.21
Biochemical parameters
	AST(U/L)	127.50 ± 29.10	172.50 ± 30.51	142.23 ± 30.17	145.00 ± 32.71
	ALT(U/L)	32.00 ± 2.83	44.50 ± 3.54*	43.33 ± 12.58	37.50 ± 3.54*
	Creatinine (mg/dL)	0.53 ± 0.11	0.70 ± 0.11	0.55 ± 0.05	0.55 ± 0.09

Organ coefficients (liver, kidney, and spleen) for DBA/1J mice treated with saline, MTX (3 mg/kg of body weight; injected every third day from day 22 to day 42 after 2nd booster injection) or brazilin (10 mg/kg of body weight; injected daily from day 22 to day 42 after the 2nd booster injection). Statistical analysis was performed with a 2-sample t-test comparing each sample group to the related normal group. Note: *denotes statistical significance at p < 0.05 versus normal group.

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