Figure 3

Effect of fraction F2 and of subfraction F2.4 fromStryphnodendron adstringenson dispersion cells from biofilms ofCandida albicansATCC 10231. The metabolic activity of dispersion cells was estimated using an XTT assay, using dispersion cells collected from biofilms under formation (A – C) or mature (formed for 24h at 35°C, prior to the start of treatment) (D – F). Biofilms were treated for 24 or 48h at 35°C with fluconazole (A and D), F2 (B and E) or F2.4 (C and F). Dispersion cells from biofilms treated with amphotericin B were not analyzed, since these were found in insufficient numbers to allow accurate quantification. The error bar represents the standard error of the mean. **p < 0.01; ***p < 0.0001 (compared to untreated biofilms, using the Dunnett’s test).