Effect of fraction F2 and of subfraction F2.4 fromStryphnodendron adstringenson mature biofilms ofCandida albicansATCC 10231. The metabolic activity of biofilm sessile cells was quantified using an XTT assay (A–D), and the total biofilm biomass was quantified using a cristal violet-based assay (E–H). Biofilms formed for 24h at 35°C were treated for 24 or 48h, at 35°C (and under agitation) with amphotericin B (A and E), fluconazole (B and F), F2 (C and G) or F2.4 (D and H). The error bar represents the standard error of the mean. *p < 0.05; **p < 0.01; ***p < 0.0001 (compared to untreated biofilms, using the Dunnett’s test).