Figure 4

K02, K03, K04, and K06 induce the expression of apoptosis-related genes in the HBL-100 cell line. (A) After treating HBL-100 cells with 10 μg/mL of K02, K03, K04, or K06 for 0, 12, 24, or 36 h, western blot was performed to analyze apoptosis-related proteins. β-Actin was used as the internal control for relative quantitation. Cleaved caspase-3 increased with treatment time to a peak at 24 h and decreased at 36 h under K02 and K06 treatment. Cleaved PARP also increased with treatment time to a peak at 24 h under K02 and K06 treatment. Bax expression was significantly increased at 36 h with all four compounds. Bcl-x expression decreased with treatment time, though without significance.(B) After treating HBL-100 cells with 10 μg/mL of K02, K03, K04, or K06 for 0, 12, 24, or 36 h, western blot was performed to analyze p53, p21, p-AKT and total AKT. p53 and p21 expression were induced after treatment with K02, K03 or K06 and increased with treatment time with all four compounds. p-AKT progressively decreased after treatment with K02 and K06, while total AKT showed no changes.