Figure 3

The effects of SMYGT on FAK signaling and MMP2 expression in HUVECs. (A) Time-(left, 200 μg/mL of SMYGT) and dose-(right, 24 h) dependent effect of SMYGT on phosphorylation of the intracellular FAK protein were determined using western blot analyses. β-actin was used to confirm equal loading of total proteins. (B) Time-(left, 200 μg/mL of SMYGT) and dose-(right, 24 h) dependent changes of MMP2 enzyme activities and mRNA induced by SMYGT were determined by zymography (top) and qPCR (bottom), respectively. A PAGE gel stained with Coomassie blue solution (middle) is presented to demonstrate equal loading of the proteins for the MMP2 zymography. V, vehicle; S, SMYGT.