Figure 2

GA induces XBP1 mRNA splicing in HeLa cells. HeLa cells were treated with 10 μg/ml of tunicamycin (Tm) or 0.3 μg/ml of GA for 0, 4 and 8 h. mRNA was extracted and subjected to the RT-PCR. The active form was normalized to the inactive form at 0 h. Results are mean values ± SD of three independent experiments (n = 3). *p < 0.05; **p < 0.01 shows significant difference compared with 0 h; #p < 0.05, is significantly different as compared between 4 h and 8 h.