Effects of an inhibitor of HO-1 on SHME-mediated attenuation of ROS formation and apoptosis induction by H
in C2C12 cells. (A) Cells grown under the same conditions as those in Figure 6 were assayed for ROS generation by DCF fluorescence. (B) The degree of apoptosis was evaluated by sub-G1 DNA content using a flow cytometer. (C) Cell viability was estimated by the MTT assay. Data are presented as the mean ± SEM, obtained from three independent experiments (*P < 0.05, compared with the control group; #P < 0.05, compared with the H2O2-treated group; $P < 0.05, compared with the H2O2 and SHME-treated group).