Exposure of SCp2 cells to Y
at noncytotoxic concentrations reduces, in a concentration-dependent manner, ET-induced IL-6 levels. (A) SCp2 cells were treated on day 3 of culture with EtOH (Ctrl) or Y2+3 at different concentrations in DM supplemented with 1% FBS. Forty-eight hr later, trypan blue assay was performed. SCp2 cells were treated (B) on day 3 of culture 30 min before ET (short-term exposure) or (C) for 3 days (long-term exposure), as of day 1 of culture, with EtOH (Ctrl) or Y2+3 at different concentrations. Cells were treated on day 3 with ET at 10 μg/ml in DM supplemented with 1% FBS and their media collected 9 hr post-ET. The values depicted are the means (± S.D.) of a triplicate treatment. Means with the same letter are not significantly different. Statistical significance of the difference from Ctrl+ET is with **P < 0.01 or ***P < 0.001. (Ctrl, control; ET, endotoxin; IL-6, interleukin-6). -ET (white square); +ET (black square).