Figure 5
![Figure 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1472-6882-8-60/MediaObjects/12906_2008_Article_219_Fig5_HTML.jpg)
Effect of Petiveria alliacea F4 fraction on cell cycle. A. A375 cells treated with ethanol (0.2%), or F4 fraction (31.2 μg/ml) or vincristine (0.1 μg/ml) for 24 h, were permeabilized, stained with propidium iodide (PI) 50 μg/ml and analyzed through flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Bars represent relative percentage of cell-cycle distribution ± SEM and represent three independent performed experiments. ***p < 0.001 versus control (ethanol 0.2%; Unpaired Student's t-test). B. A375 cells treated with ethanol (0.2%) or F4 fraction (31.2 μg/ml) or vincristine 0.1 μg/ml for 12, 18, 24 and 48 h, were permeabilized, stained with PI 50 μg/ml and analyzed through flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Histograms represent relative cell DNA content representing two independent experiments. C. A375 cells treated with ethanol (0.2%) or F4 fraction(62.5, 31.2, 15.6 μg/ml) or vincristine (0.1 μg/ml) for 48 h, were permeabilized, stained with PI 50 μg/ml and analyzed through flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Histograms represent relative cell DNA content representing two independent experiments.