F4 fraction induces morphological changes in tumor cells. A. A375 cells treated with ethanol 0.2% (left panel), vincristine 0.1 μg/ml (middle panel) or F4 fraction 31.2 μg/ml (right panel). Morphological changes were analyzed under invert microscope. Results represent three independent performed experiments. B. A375 (104) treated with ethanol 0.2% (left panel) or F4 fraction 31.2 μg/ml (middle and right panels) for 24 h. Cells were stained with Oregon Green-phalloidin were analyzed under fluorescent microscope. Results show photos representing four independent experiments.