MAK-4 and -5 supplemented diet inhibits liver carcinogenesis in mice

Table 4 Dosage effect of MAK-4 and MAK-5 on the growth of transformed Rat 6 cells

R6/T24 is a permanent cell line transformed by an activated human c-H-ras oncogene. Both normal and H-ras transformed Rat 6 cells were seeded in triplicate at 10⁴ cells per 60 mm plate in D10CS. Next day, cultured were switched to D5FCS, or FCS plus herbal extracts at designated dosages for 5 days. At the end of the treatment, cells were trypsinized and counted by Coulter Counter. Cytotoxicity was expressed as percent of cell survival compared to the untreated control.

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