Figure 3

Effects of S-A144 on the PDGF-induced activation of ERK1/2, Akt and PLCγ1. Quiescent VSMCs cultured in serum-free medium were stimulated with 25 ng/mL PDGF-BB, followed by the addition of various concentrations of S-A144 (100–500 μg/mL) or S-AOR (500 μg/mL) to determine the effect on phosphorylation of ERK1/2, Akt and PLCγ1, as measured by SDS-PAGE and immunoblotting. Total ERK1/2, Akt or β-actin were used for normalisation. Data are representative of three independent experiments. Values are expressed as the mean ± S.E.M. Statistically significant differences relative to PDGF controls (PDGF-stimulated) are indicated by **P < 0.01.