Figure 3

Impact of Zyflamend on mRNA and protein expression of p21 and p27. Cells were treated with increasing concentrations of Zyflamend (0, 40, 100, 150 and 200 μg/mL). (A) Expression of mRNA levels. (B) Expression of protein levels. The bar graph quantitates the relative protein level of p21 ± Zyflamend at 0 and 200 μg/mL. mRNA and protein values are mean ± SEM, n = 3. Values are significantly different from non-treatment: *p < 0.05; **p < 0.01.