Figure 4

Induction of apoptosis in MCF-7 cells by EADs determined using Annexin V-PI flowcytometry technique. The data representing three independent tests which displayed similar results. The lower left quadrant represents intact viable cells (Annexin-FITC and PI negative). The lower right quadrant represents early apoptotic cells (Annexin-FITC positive and PI negative). The upper right region represents late apoptotic cells or secondary necrotic cells (Annexin-FITC and PI positive). The data are presented as dot plots of Annevin V/FITC against PI of at least three independent tests.