Figure 2
Effect of EBT on cell viability, β-Hex release, LTC 4 and PGD 2 productions. (A) BMMC were treated with different concentrations of EBT and the cell viability was measured. BMMC were pre-incubated with the indicated concentrations of EBT for 1 hr and stimulated with KL (100 ng/ml) for 15 min. Levels of (B) β-Hex and (D) LTC4 released into the supernatant were determined. (C) BMMC were pre-incubated with 10 μg/mL of aspirin for 2 h to abolish pre-existing COX-1 activity, followed by washing and then stimulation with KL/IL-10/LPS for 7 h. PGD2 released into the supernatant was quantified. *: P < 0.05, **: P < 0.01, and ***: P < 0.001 significantly different from AR mice as determined by a one-way ANOVA.