Effects of EAW on lipid peroxidation, TUNEL assay, and neuronal density in the cortext of D-galactose-treated mice. (A) MDA levels were analyzed as described in the text. (B) Representative of TUNEL stained brain section. Arrow indicates TUNEL positive cells. (C) TUNEL positive cells were quantified as described in the text. Data are expressed as mean ± SD (n = 6). (D) Representative hematoxylin-eosin stained sections of the cortex in each group (200×). Scale bar: 100 μm. (E) Neuronal density calculated from the number of neurons in a 0.218 mm2 field (400x) and expressed as the mean ± SD for the number of mice indicated in the text. # P < 0.05, compared to the control group; *P < 0.05 and **P < 0.01, compared to the D-galactose-treated group.