Mechanistic basis for protection of differentiated SH-SY5Y cells by oryzanol-rich fraction against hydrogen peroxide-induced neurotoxicity

Ismail, Norsharina; Ismail, Maznah; Imam, Mustapha Umar; Azmi, Nur Hanisah; Fathy, Siti Farhana; Foo, Jhi Biau; Abu Bakar, Muhammad Firdaus

doi:10.1186/1472-6882-14-467

BMC Complementary Medicine and Therapies

Table 1 Gene name, accession number, reverse and forward primer sequences used in GeXP multiplex gene expression analysis

From: Mechanistic basis for protection of differentiated SH-SY5Y cells by oryzanol-rich fraction against hydrogen peroxide-induced neurotoxicity

Gene name	Accession number	Primer sequences* with universal tags
		Forward	Reverse
Antioxidant genes
Catalase	NM_001752	AGGTGACACTATAGAATA GAAGTGCGGAGATTCAACACT	GTACGACTCACTATAGGGA ACACGGATGAACGCTAAGCT
SOD 1	NM_000454	AGGTGACACTATAGAATATCA TCAATTTCGAGCAGAAGG	GTACGACTCACTATAGGGA TGCTTTTTCATGGACCACC
SOD 2	NM_000636	AGGTGACACTATAGAATA CATCAAACGTGACTTTGGTTC	GTACGACTCACTATAGGGA CTCAGCATAACGATCGTGGTT
Downstream apoptotic genes
BAD	NM_004322	AGGTGACACTATAGAATA CGGAGGATGAGTGACGAGTT	GTACGACTCACTATAGGG AGGAGTTTCGGGATGTGGAG
TNF	NM_000594	AGGTGACACTATAGAATA CTATCTGGGAGGGGTCTTCC	GTACGACTCACTATAGGG AATGTTCGTCCTCCTCACAGG
ING3	NM_198267	AGGTGACACTATAGAATA AGGTTCAGTTGGCAAACCAG	GTACGACTCACTATAGGG AAGCCAAAAGTGAGCATGTGTT
BAK1	BC004431	AGGTGACACTATAGAATA AGCCTGTTTGAGAGTGGCAT	GTACGACTCACTATAGGG AAGTGATGCAGCATGAAGTCG
BAX	BC014175	AGGTGACACTATAGAATA CCCTTTTGCTTCAGGGTTTC	GTACGACTCACTATAGGG ACAAAGTAGAAAAGGGCGACAA
p21	NM_000389	AGGTGACACTATAGAATA TTAGCAGCGGAACAAGGAGT	GTACGACTCACTATAGGG AAGCCGAGAGAAAACAGTCCA
CAS-9	NM_001229	AGGTGACACTATAGAATA GGGCTCACTCTGAAGACCTG	GTACGACTCACTATAGGG ATCTGGAAGCTGCTAAGAGCC
BCL-2	M14745	AGGTGACACTATAGAATA ACCACTAATTGCCAAGCACC	GTACGACTCACTATAGGG ATTTTCCATCCGTCTGCTCTT
Upstream apoptotic genes
ERK1/2	NM_002745	AGGTGACACTATAGAATA GGAGCAGTATTACGACCCGA	GTACGACTCACTATAGGGA GATGTCTGAGCACGTCCAGT
p53	NM_001126117	AGGTGACACTATAGAATA GGGGAGCAGGGCTCA	GTACGACTCACTATAGGGA AAAATGGCAGGGGAGGG
JNK	NM_139046	AGGTGACACTATAGAATA CAGAAGCTCCACCACCAAAGAT	GTACGACTCACTATAGGGA GCCATTGATCACTGCTGCAC
PARP1	NM_001618	AGGTGACACTATAGAATA TATCGAGTCGAGTACGCCAA	GTACGACTCACTATAGGGA GTGTGGGACTTTTCCATCAAA
AKT1	NM_001014431	AGGTGACACTATAGAATA GAGGAGATGGACTTCCGGTC	GTACGACTCACTATAGGGA AGGATCTTCATGGCGTAGTAGC
NF-kB	NM_001077493	AGGTGACACTATAGAATA GCGGGCGTCTAAAATTCTG	GTACGACTCACTATAGGG ATTCCACGATCACCAGGTAGG
p38	NM_001315	AGGTGACACTATAGAATA TTCAGTCTTTGACTCAGATGCC	GTACGACTCACTATAGGGA GTCAGGCTTTTCCACTCATCT
Housekeeping genes
GAPDH ^a	NM_002046	AGGTGACACTATAGAATA AAGGTGAAGGTCGGAGTCAA	GTACGACTCACTATAGGGA GATCTCGCTCCTGGAAGATG
Hyaluronidase ^a	AJ000099	AGGTGACACTATAGAATA CAGCAGTTCATGCTGGAGAC	GTACGACTCACTATAGGGA CCAGGTAGACAGACGGGAAG
18 sRNA ^a	M10098	AGGTGACACTATAGAATA GGAGTGGAGCCTGCGGCTTAA	GTACGACTCACTATAGGGA TAGCATGCCAGAGTCTCGTT
Actb ^a,#	NM_001101	AGGTGACACTATAGAATA GATCATTGCTCCTCCTGAGC	GTACGACTCACTATAGGGA AAAGCCATGCCAATCTCATC
Kan(r) ^b	-	AGGTGACACTATAGAATA ATCATCAGCATTGCATTCGATTCCTGTTTG	GTACGACTCACTATAGGGA ATTCCGACTCGTCCAACATC

*Based on the Homo sapien gene sequences adopted from the National Center for Biotechnology Information GenBank Database.
^aHousekeeping genes; ^bInternal control; ^#Normalization gene.

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ISSN: 2662-7671

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