Figure 3

Inhibitory effect of EEOS on invasion ability of NCI-H460 cells mediated by osteopontin. Effect of EEOS in the absence or presence of OPN (50 nM) on the invasion ability of NCI-H460 cells. The cells pretreated for 7 h with or without EEOS (0, 100 or 200 μg/ml), were placed in the upper well of the migration chamber and incubated at 37°C for 6 h. The filter was then removed and fixed in methanol. At the end of incubation, images of a number of invaded cells were taken under a light microscope per each field (A) and finally quantified (B). *Statistically significant value compared with control data (**p < 0.01).