Palm kernel cake extract exerts hepatoprotective activity in heat-induced oxidative stress in chicken hepatocytes

Table 1 The primer characteristics used for the gene expression analysis

Genes		Sequences (5′ to 3′)	References
TNF-like¹	F	tgctgttctatgaccgcc	[20]
TNF-like¹	R	ctttcagagcatcaacgca	[20]
IL-1β²	F	atg gcgttcgttcccgacctggacgtgctg	[21]
IL-1β²	R	acttagcttgtaggtggcgatgttgacctg	[21]
IFN-γ³	F	gctgacggtggacctattatt	[21]
IFN-γ³	R	tggattctcaagtcgttcatcg	[21]
GAPDH⁴	F	tgaaagtcggagtcaacggatt	[22]
GAPDH⁴	R	ccacttggactttgccagaga	[22]
β-Actin	F	caacacagtgctgtctggtgg	[20]
	R	atcgtactcctgcttgctgat	[20]

¹In chickens, TNF-α has not been identified. but TNF-like ligand 1A (TNF-like) produced effects similar to TNF-α.
²Interleukin-1 beta.
³Interferon gamma.
⁴Glyceraldehyde 3-phosphate dehydrogenase.

ISSN: 2662-7671