Figure 5

Effect of GV20 acupuncture stimulation on the percentage values of the mean (±standard errer, SE) number of choline acetyltransferase (ChAT)-stained neurons and acetylcholinesterase (AchE)-stained neurons in various hippocampal areas after the Morris water maze (MWM) task (n = 3-4/group). Representative photographs and the relative percentage values are shown in (A) and (B and C), respectively. (a): ChAT expression in the SAL group, (b): ChAT expression in the SCO group, (c): ChAT expression in the SCO + GV20 group, (d): AchE expression in the SAL group, (e): AchE expression in the SCO group, (f): AchE expression in the SCO + GV20 group. Sections were cut coronally at 30 μm. Scale bar represents 50 μm. ^* p < 0.05, ^** p < 0.01 vs. the SAL group; ^# p < 0.05 vs. the SCO group.