Experimental schedules for scopolamine (SCO) administration to induce spatial memory impairments in rats. The experiment was designed to explore the efficacy of acupuncture therapy in healing chronic SCO-induced spatial memory impairment in an animal model using behavioral and neurobiological methodologies. All rats excluding the SAL group received SCO injection. Two weeks after SCO injection, the rats were subjected to the Morris water maze (MWM) and the passive avoidance test (PAT). After behavioral testing, rats were sacrificed and brain tissues was collected immediately. SCO: scopolamine; IHC: Immunohistochemistry.