Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

Table 2 Anticlastogenic effect of Darjeeling tea extract (DTE) against benzo[a]pyrene (B[a]P) in human lymphocyte culture as measured by micronuclei formation

Clastogen (μM)	DTE (μg/ml)	Number of micronucleated cells/1000 binucleated cells (mean + _S.D.)
-	25	1.33 ± 1.22
-	50	1.27 ± 0.98
-	100	1.35 ± 1.08
-	200	2.63 ± 1.46
B[a]P 100	-	18.69 ± 3.39
B[a]P 100	25	15.62 ± 2.37 *
B[a]P 100	50	12.56 ± 2.77 **
B[a]P 100	100	8.07 ± 1.68 **
B[a]P 100	200	10.49 ± 3.12 **

Each data represented as Mean ± SD of six samples per concentration. Results at of each concentration of DTE plus positive mutagen benzo[a]pyrene (B[a]P) were compared with the positive mutagen treated group alone (all in presence of S9 mix) by Dunnett’s multiple comparison. *P < 0.05, **P < 0.01.

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