CGN PE extract treatment induces apoptosis in human breast cancer cells. (A) ZR751 cells were treated with (5, 10 and 20 μg/ml) or without PE extract for 24 h prior to stain with AO/EB and viewed under fluorescence microscope (400×). The treated ZR751 cells showed characteristic features of apoptosis such as nuclear condensation (white arrow) and nuclear fragmentation (blue arrow). (B) ZR751 cells were treated with (5, 10 and 20 μg/ml) or without PE extracts for 24 h prior to DNA fragmentation assay. The lane NC, PC, 5, 10, 20 and M represent treatment with negative control, positive control (curcumin), 5 μg/ml PE extract, 10 μg/ml PE extract, 20 μg/ml PE extract, and marker (1 kb DNA ladder), respectively.