Effects of SAMC on the activities of caspases, FADD and PARP. Effects of SAMC on the activities of caspase-3/7, caspase-9 and caspase-8 in human breast cancer cell lines MCF-7 and MDA-MB-231 are shown in (A), (B) and (C), respectively. (D) The levels of caspase-7, PARP and FADD were measured by Western blot analysis. MCF-7 and MDA-MB-231 cells were exposed to various concentrations of SAMC. The values of optical density (OD) at 405 nm were determined in triplicate and all values are presented as mean ± S.D. of three independent experiments, *p < 0.05 vs. control cells.