The inhibitory effects and cell cycle progression of SAMC on human breast cancer cells. The experiments were performed in triplicate and data are presented as mean ± S.D. of three independent experiments, *p < 0.05 when compared with control group. (A) Cytotoxic effects of SAMC on breast cancer cells for 24, 48 and 72 h measured by the SRB assay. (B) Cells were treated with SAMC (0–600 μM) for 24 h. For cell cycle analysis, cells were fixed, stained with PI and analyzed by flow cytometry. Quantitative analysis of percentage gated cells at G0/G1, S and G2/M phases were shown. All values were expressed as mean ± S.D. (C) Cell cycle analysis by flow cytometry, a hallmark of apoptosis was noted at high concentration of 400 and 600 μM.