Bax, Bcl-2, Caspase-9, and −3 expression level. Bax and Caspase-3 were activated while Bcl-2 and Caspase-9 were suppressed conspicuously after FFAs 1 mM treatment in HepG2 cells. But, AC extract treatment for 24 h reduced the expression level of Bax and Caspase-3, and augmented the Bcl-2 and Caspase-9 manifestation (A). In a bar graph, AC treatment suppressed the Bax/Bcl-2 expression level which had been significantly increased after induction of lipoapoptosis (B). The cleaved forms of Caspase-3 and Caspase-9 were down-regulated after treatment of AC extract (A). Statistical significance was determined by one-way ANOVA and the values are mean ± SEM. ###, p < 0.001, control versus FFAs-treated group (lipoapoptosis-induced group) and ***, p < 0.001, FFAs-treated group versus AC extract treated group.