PUMA mRNA expression level. PUMA mRNA was prepared from control cells, cells treated with FFAs 1 mM for 24 h, and cells pretreated with FFAs 1 mM for 24 h and then cultured with AC extract for 24 h, respectively. Data were normalized with GADPH level. Statistical significance was determined by one-way ANOVA and the values are mean ± SEM. ###, p < 0.001, control versus FFAs-treated group (lipoapoptosis-induced group) and ***, p < 0.001, FFAs-treated group versus AC extract treated group.