Cell viability assay. After treatment of AC extract and FFAs 1 mM on the HepG2 cells, MTT assay was performed. AC extract was treated as 100, 500 and 1000 μg/ml for 24 h. AC 100 μg/ml showed no toxicity to HepG2 cells (A). Differently concentrated AC extracts were treated on HepG2 cells for 24 h and the LD50 was calculated (B). FFAs were treated at 1 mM concentration for 1 h or 24 h. The treatment of FFAs showed significant toxicity to HepG2 cells for both 1 h or 24 h (C). Statistical significance was determined by one-way ANOVA and the values are mean ± SEM. **, p < 0.01; ***, p < 0.001.