Figure 6
Fluorescence staining showing sperm DNA integrity in each group. The sperm head was fluorescent green indicating normal DNA (red arrow), and red or yellow fluorescence reflecting to abnormal DNA (red arrow). The number of sperm cells with normal DNA was significantly lower in the natural aging group (B), while the number of sperm cells with normal DNA was higher in the young control group (A), the Shouwu pill group (D), Heshouwuyin group 1 (E), and Heshouwuyin group 2 (C), compared with the natural aging group. Scale bar, 100 μm.