Table 1 Primer sequences used for quantitative real-time PCR in this study
Gene | (GenBank accession no.) | Sequence (5’–3’) |
|---|---|---|
Asbt | XM_003751634 | GGATAGATGGTGACATGGAC (fw) |
GAAACAGGAATAACAAGCGC (rev) | ||
Bsep | U69487 | CTGATGCTTATGGGAGGCGTAT (fw) |
TCTGGTGGAAGGAGCTGTTGATC (rev) | ||
Cyp2b1 | NM_001134844 | GCTATGGTTTCCTGCTGATGCTC (fw) |
GACTCTGTGTGGTACTCCAATAGGG (rev) | ||
Mdr2 | NM_012690 | GTTGAAATGAAAATGTTGGCTGG (fw) |
ATGCCGTAGATGTGAGCCTTCC (rev) | ||
Mrp2 | NM_012833 | TCAGATGAGGAGGTTTGGAGGG (fw) |
CTCTGTCACTTCGGATAACAACCC(rev) | ||
Mrp3 | NM_080581 | TGAAGAGACTGGAATCCGTTAGCC (fw) |
GTTGGAGGCGATGTAAGGATAAGTG (rev) | ||
Mrp4 | NM_133411 | GACACCAAAAACGCGAACGG (fw) |
ACAAGGACATAGAACACCAGCAGG (rev) | ||
Ntcp | NM_017047 | CTACCTCCTCCCT GATGCCCTT (fw) |
TGAGGATGGTAGAACAGAGTTGAATG (rev) | ||
Oatp1 | NM_017111 | TGCCTGCCTTCTTCATTCTGATACT (fw) |
GTTTTCAGTTCTCCGTCATTCTCG (rev) | ||
Ostα | NM_001107087 | TCCTCTGTCCCTGAGCCTTATC (fw) TCTCTGCTTTCACCATTTATTACAA (rev) |
Ostβ | XM_238546 | TGCTTTGGTATTTCCGTTC (fw) |
TCTGGCATTCCGTTGTCTT (rev) | ||
Sult2a1 | NM_131903 | ATCAAAAAGAAAGGACCACGACTC (fw) |
AACCATTCAACATAAGTTCCCAGTG (rev) | ||
Ugt1a1 | NM_012683 | TGAAGCCTATGTCAACGCCTC (fw) |
CGATGGTCTAGTTCCGGTGTAGC (rev) | ||
Gapdh | NM_017008 | AGAAGGTGGTGAAGCAGGCATC (fw) |
CGAAGGTGGAAGAGTGGGAGTTG (rev) |