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Table 2 Primers used for RT-PCR analysis

From: Oryeongsan inhibits LPS-induced production of inflammatory mediators via blockade of the NF-kappaB, MAPK pathways and leads to HO-1 induction in macrophage cells

Target gene

Primer sequence

Annealing temp

TNF-α

F: 5′-AGCACAGAAAGCATGATCCG-3′

55°C

 

R: 5′-GTTTGCTACGACGTGGGCTA-3′

IL-6

F: 5′-CATGTTCTCTGGGAAATCGTGG-3′

58°C

 

R: 5′-AACGCACTAGGTTTGCCGAGTA-3′

IL-1β

F: 5′-TGCAGAGTTCCCCAACTGGTACATC-3′

64°C

 

R: 5′-GTGCTGCCTAATGTCCCCTTGAATC-3′

COX-2

F: 5′-CACTCAGTTTGTTGAGTCATTC-3′

45°C

 

R: 5′-GATTAGTACTGTAGGGTTAATG-3′

iNOS

F: 5′-AGCCCAACAATACAAATGACCCTA-3′

56°C

 

R: 5′-TTCCTGTTGTTTCTATTTCCTTTGT-3′

HO-1

F: 5′-TGAAGGAGGCCACCAAGGAGG-3′

62°C

 

R: 5′-AGAGGTCACCCAGGTAGCGGG-3′

β-actin

F: 5′-ATGAAGATCCTGACCGAGCGT-3′

58°C

 

R: 5′-AACGCAGCTCAGTAACAGTCCG-3′

  1. F, forward; R, reverse.