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Table 2 Primers used for RT-PCR analysis

From: Oryeongsan inhibits LPS-induced production of inflammatory mediators via blockade of the NF-kappaB, MAPK pathways and leads to HO-1 induction in macrophage cells

Target gene Primer sequence Annealing temp
TNF-α F: 5′-AGCACAGAAAGCATGATCCG-3′ 55°C
  R: 5′-GTTTGCTACGACGTGGGCTA-3′
IL-6 F: 5′-CATGTTCTCTGGGAAATCGTGG-3′ 58°C
  R: 5′-AACGCACTAGGTTTGCCGAGTA-3′
IL-1β F: 5′-TGCAGAGTTCCCCAACTGGTACATC-3′ 64°C
  R: 5′-GTGCTGCCTAATGTCCCCTTGAATC-3′
COX-2 F: 5′-CACTCAGTTTGTTGAGTCATTC-3′ 45°C
  R: 5′-GATTAGTACTGTAGGGTTAATG-3′
iNOS F: 5′-AGCCCAACAATACAAATGACCCTA-3′ 56°C
  R: 5′-TTCCTGTTGTTTCTATTTCCTTTGT-3′
HO-1 F: 5′-TGAAGGAGGCCACCAAGGAGG-3′ 62°C
  R: 5′-AGAGGTCACCCAGGTAGCGGG-3′
β-actin F: 5′-ATGAAGATCCTGACCGAGCGT-3′ 58°C
  R: 5′-AACGCAGCTCAGTAACAGTCCG-3′
  1. F, forward; R, reverse.