Table 1 Primer sequence and condition for RT-PCR
Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Condition | PCR product length (bp) |
|---|---|---|---|---|
β-actin | TGGAATCCTGTGGCATCCA | TAACAGTCCGCCTAGAAGCA | 95°C 3 min, (94°C 45 s, 55°C 45 s, 72°C 45 s) for 32 cycles, 72°C 10 min | 343 |
IL-6 | GAGGATACCACTCCCAACAGACC | AAGTGCATCATCGTTGTTCATACA | 95°C 5 min, (94°C 30 s, 58°C 30 s, 72°C 60 s) for 35 cycles, 72°C 10 min | 141 |
TNF-α | GGCAGGTCTACTTTGGAGTCATTGC | ACATTCGAGGCTCCAGTGAATTCGG | 95°C 5 min, (94°C 30 s, 58°C 30 s, 72°C 60 s) for 35 cycles, 72°C 10 min | 300 |
RACK1 | ACCAACAAGGCGATTTGTCG | GCAGACACCCAGAGTATTCCATA | 94°C 2 min, (94°C 30 s, 52°C 30 s, 72°C 2 min) for 32 cycles, 72°C 8 min | 136 |
BDNF | AGCCTCCTCTGCTCTTTCTGCTGGA | CTTTTGTGTATGCCCCTGCAGCCTT | 95°C 5 min, (95°C 45 s, 58°C 45 s, 72°C 60 s) for 32 cycles, 72°C 10 min | 298 |