Gene expressions and collagen production in NIH/3T3 fibroblasts with or without TGF- β1 and/or Yiqihuoxue treatment. (A) Relative transcript levels of Col1a2, Col3a1, Ctgf, Sparc and Tgf-β1 in murine 3T3 fibroblasts with different treatments. N, non-treated; T, TGF-β1; Y, Yiqihuoxue decoction. The expression level of each gene in non-treated murine 3T3 fibroblasts was normalized to 1. *, P <0.05; **, P <0.001. (B) Western blotting analysis of type I collagen (COL1) in murine 3T3 fibroblasts with different treatments. (C) Densitometric analysis of western blotting for type I collagen (COL1). Bars showed the mean ± SD results of three assays. *, P <0.05; **, P <0.001.