Inhibition of hematopoietic prostaglandin D2 Synthase (H-PGDS) by an alkaloid extract from Combretum molle

Table 1 The effects of alkaloids from C. molle on the kinetic properties of GST Sigma

[ *C.molle*]	K_cat(S^-1)	K_cat(S^-1)	K_m(mM)	K_m(mM)	K_cat/K_m(S^-1 mM^-1)	K_cat/K_m(S^-1 mM^-1)
(μg/ml)	GSH	CDNB	GSH	CDNB	GSH	CDNB
0	171.2 ± 1.7	2366.8 ± 38.6	5.2 ± 0.04	2.25 ± 0.04	33.17 ± 0.06	1051.2 ± 1.03
2	162 ± 0.3	2281.3 ± 3.5	4.82 ± 0.12	2.00 ± 0.02	33.62 ± 0,80	1136.14 ± 6.70
5	149.5 ± 2.6	2218.6 ± 37.3	3.41 ± 0.007*	2.17 ± 0.007	43.82 ± 0.86	1020.19 ± 13.83
10	127.1 ± 5.3*	2118.4 ± 31.2	1.40 ± 0.02**	2.06 ± 0.02**	90.92 ± 4.81	1028.83 ± 5.96
20	115.6 ± 1.9***	2074.5 ± 3.2*	1.14 ± 0.06**	2.02 ± 0.02**	101.9 ± 3.94	1029.36 ± 10.33

One-way analysis of variance test (ANOVA) with Dunnett’s Multiple Comparison Test was used to analyse the results. Values represent the mean ± SD for N = 2. The values with a p-value < 0.05 or less were considered statistically significant. *P < 0.05, **P < 0.01 , ***P < 0.001. Graphical and Statistical analyses were carried out using Graphpad Prism 5® Software (Version 5.0, Graph pad Software Inc, San Diego, USA).

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