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Table 1 The effects of alkaloids from C. molle on the kinetic properties of GST Sigma

From: Inhibition of hematopoietic prostaglandin D2 Synthase (H-PGDS) by an alkaloid extract from Combretum molle

[ C.molle] Kcat(S-1) Kcat(S-1) Km(mM) Km(mM) Kcat/Km(S-1 mM-1) Kcat/Km(S-1 mM-1)
(μg/ml) GSH CDNB GSH CDNB GSH CDNB
0 171.2 ± 1.7 2366.8 ± 38.6 5.2 ± 0.04 2.25 ± 0.04 33.17 ± 0.06 1051.2 ± 1.03
2 162 ± 0.3 2281.3 ± 3.5 4.82 ± 0.12 2.00 ± 0.02 33.62 ± 0,80 1136.14 ± 6.70
5 149.5 ± 2.6 2218.6 ± 37.3 3.41 ± 0.007* 2.17 ± 0.007 43.82 ± 0.86 1020.19 ± 13.83
10 127.1 ± 5.3* 2118.4 ± 31.2 1.40 ± 0.02** 2.06 ± 0.02** 90.92 ± 4.81 1028.83 ± 5.96
20 115.6 ± 1.9*** 2074.5 ± 3.2* 1.14 ± 0.06** 2.02 ± 0.02** 101.9 ± 3.94 1029.36 ± 10.33
  1. One-way analysis of variance test (ANOVA) with Dunnett’s Multiple Comparison Test was used to analyse the results. Values represent the mean ± SD for N = 2. The values with a p-value < 0.05 or less were considered statistically significant. *P < 0.05, **P < 0.01 , ***P < 0.001. Graphical and Statistical analyses were carried out using Graphpad Prism 5® Software (Version 5.0, Graph pad Software Inc, San Diego, USA).