Figure 6

Intracellular inhibition of HCV replication in infected Huh 7.5 cells by cLF (A), N-lobe (B) and C-lobe (C). HCV infected Huh 7.5 cells were treated with cLF, N-lobe or C-lobe for 96 h. RT nested PCR was performed to amplify viral RNA segments. Lane 1, DNA ladder; lane 2, negative control; lane 3, positive control; lane 4-8, proteins at different concentrations, as indicated under the gel graph. The upper arrow pointed the Rulc which served as internal control, while the lower arrow pointed the positive amplified 1740 bp of HCV.