The percentage of viable, apoptotic and necrotic/secondary necrotic cells of untreated and DCM-DS-treated MCF-7 cells for 24 and 48 hours as determined by flow cytometry. (A and B) These figures are from representative experiments carried out at least three times. The percentage of viable cells was represented by the lower left quadrant (Annexin-V-/PI-); the percentage of early apoptotic and necrotic/secondary necrotic cells were represented by the lower right (Annexin-V+/PI-) and upper (PI+) quadrants, respectively. Each data point represents the mean of three independent experiments ± SD. *significantly different from the control (p < 0.05).