Figure 5

Effects of TCE and ERK1/2 inhibitor (U0126) on u-PA expression, in vitro wound closure, cell migration, and invasion in Huh7 cells. (A-B) Huh7 cells were pretreated with U0126 for 30 min and then incubated in the presence or absence of TCE for 24 h. The cell lysates were then subjected to SDS–PAGE followed by western blots with anti-u-PA antibodies, as described in the Methods Section. (C-E) Huh7 cells were pretreated with U0126 for 30 min and then incubated in the presence or absence of TCE for 24 h. The Huh7 cells were then subjected to in vitro wound closure, cell migration, and invasion assay. The migration and invasion abilities of Huh7 cells were quantified by counting the number of cells that invaded the underside of the porous polycarbonate, as described in the Methods Section. The values in this figure represent the means ± SD of at least 3 independent experiments. * p < 0.05 as compared with the vehicle group.