Figure 3

The critical role of NF-κB and SP-1 in TCE -induced transcriptional inhibition of u-PA in Huh7 cells. Huh7 cells were treated with TCE 100 μg/mL for 24 h and the nuclear fraction was prepared as described in the Methods Section. This figure shows the ChIP analysis of the association of (A-B) NF-κB and (C-D) SP-1 transcription factors with the u-PA promoter region in Huh7 cells. The values in this figure represent the means ± SD of at least 3 independent experiments. *p < 0.05 as compared with the vehicle group.