Figure 2

Immunohistochemistry for detection of Ang-1 and Ang-2 after ICH. After ICH, some Ang-1- (A) and Ang-2- (B) positive microvessels of the enlarged profile were detected in the perihematomal tissue after ICH. Ang-1 immunoreactivity increased notably as long as 2 weeks after ICH (C), while Ang-2 immunoreactivity declined at about 7 days following a striking upregulation at 3 days (D). EA at the Zusanli (ST36) acupoint increased Ang-1 and Ang-2 expression as compared with no EA treatment after ICH. Meanwhile, there was no significant difference in the expression of Ang-1 and Ang-2 between the SEN group and the SNE group. Scale bar = 100 μm. *P < 0.05 vs. the SNE group. ^△ P < 0.05, ^△△ P < 0.01 vs. the former time point in the SNE group (mean ± SD, n = 5 for each time point).