Figure 1
The HPLC analysis of MEDL. A. HPLC profile of MEDL at two different wavelength; i) 254 nm, and ii) 366 nm. Attempt was made to standardize the MEDL using various pure flavonoids standard. Based on the HPLC analysis, rutin and quercitrin was detected in the extract. B. HPLC profile of MEDL at 366 nm shows four major peaks, labelled as 1, 2, 3 and 4, that was successfully separated. Each peak was represented by their respective UV-Vis spectra with λmax value of 228-349, 234-350, 254-350 and 264-347 nm, respectively. These ranges of UV-Vis spectra indicated the presence of flavonoids-types of compounds.